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STEMCELL Technologies Inc bsa/insulin/transferrin bit 9500
Bsa/Insulin/Transferrin Bit 9500, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bit+9500+(bsa%2C+insulin%2C+transferrin/bit+9500/pmc09028139-36-21-27
Average 90 stars, based on 1 article reviews
bsa/insulin/transferrin bit 9500 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Cell Culture:

Article Title: Mitochondrial DNA Mutations Determine Favourable Molecular Responses to Targeted Kinase Inhibitor Therapy and Impair Oxidative Phosphorylation
Article Snippet: .. Primary samples were cultured at 37°C and 5% CO2 in serum-free media (SFM) consisting of Iscove’s modified Dulbecco medium (Sigma-Aldrich, Missouri, United Stated) supplemented with BIT 9500 Serum Substitute (bovine serum albumin, BSA/insulin/transferrin; STEMCELL Technologies, Vancouver, Canada), 2 mM L-Glutamine (Sigma-Aldrich, St. Louis, Missouri, United States), 1 mM streptomycin/penicillin, 0.1 mM 2-mercaptoethanol, 0.02 mg/mL human Low Density Lipoprotein (STEMCELL Technologies, Vancouver, Canada). .. The SFM was then filtered and supplemented with a physiological growth factor cocktail (STEMCELL Technologies, Vancouver, Canada) consisting of 0.05 ng/mL human recombinant LIF, 1 ng/mL G-CSF and 0.2 ng/mL SCF/GM-CSF/MIP-α/IL-6.

Article Title: A novel, highly potent and selective phosphodiesterase-9 inhibitor for the treatment of sickle cell disease
Article Snippet: .. CD34 + cells were were cultured in the presence of 15% BIT 9500 [mixture of bovine serum albumin (BSA) + insulin + transferrin from Stem Cell Technologies], 100 U/mL penicillin-streptomycin, 2 mM L-glutamine, 10 ng/mL recombinant human (rh) IL-3 (Peprotech), 100 ng/mL rhIL-6 (Peprotech), and 100 ng/mL rhSCF (Peprotech) for seven days and then CD36 + cells, isolated and cultured in media containing 100 ng/mL rhSCF, 10 ng/mL rhIL-3 and 2 UI/mL erythropoetin (Cilag, France) supplemented with dimethyl sulfoxide (DMSO), 30 μM HU or 10 μM IMR-687 for five days, at which point the HbF + erythroid cells (LD−/GPA + /Band3 + ) was determined by FACS. .. HbSS-Townes mice on a 129/B6 background (Jackson Laboratory, Bar Harbor, ME, USA; 10-12 weeks old, n=7 per group) were dosed daily by gavage with vehicle (polyethylene glycol in water 1:3), 50 or 25 mg/kg of HU, or 30 mg/kg of IMR-687.

Article Title: Glioma Big Potassium Channel Expression in Human Cancers and Possible T Cell Epitopes for Their Immunotherapy
Article Snippet: .. The stem cells were cultured cells on Matrigel-coated dishes with DMEM/F12 (1:1 v/v) medium (Irvine Scientific, Irvine, CA) containing 10% BIT 9500 (BSA, insulin, transferrin; Stem Cell Technologies, Tukwila, WA), 292 μg/ml glutamine (Irvine Scientific), 40 ng/ml basic fibroblast growth factor, 20 ng/ml epidermal growth factor, and 20 ng/ml platelet-derived growth factor-AB, 100 U/ml penicillin (Irvine Scientific), 100 μg/ml streptomycin (Irvine Scientific), 50 μg/ml gentamicin (Sigma-Aldrich), 10 μg/ml ciprofloxacin (Bayer), and 2.5 μg/ml amphotericin (Life Technologies/Invitrogen, Carlsbad, CA) as previously described ( 26 ). .. For continuous expansion, half of this medium was replaced every other day, and the cultures were passaged every 7 d or when confluent using nonenzymatic cell dissociation solution (Sigma-Aldrich).

Article Title: Identification of unipotent megakaryocyte progenitors in human hematopoiesis.
Article Snippet: .. To evaluate lineage relationships, 1500 HSPCs were cultured in 96-well plates with StemSpan SFEM II medium supplemented with Megakaryocyte Expansion supplement (Catalog No. 02696, STEMCELL Technologies) containing rhTPO, rhSCF, rhIL-6, rhIL-9 and 20% BIT 9500 Serum Substitute (containing BSA, Insulin, Transferrin; STEMCELL Technologies). ..

Modification:

Article Title: Mitochondrial DNA Mutations Determine Favourable Molecular Responses to Targeted Kinase Inhibitor Therapy and Impair Oxidative Phosphorylation
Article Snippet: .. Primary samples were cultured at 37°C and 5% CO2 in serum-free media (SFM) consisting of Iscove’s modified Dulbecco medium (Sigma-Aldrich, Missouri, United Stated) supplemented with BIT 9500 Serum Substitute (bovine serum albumin, BSA/insulin/transferrin; STEMCELL Technologies, Vancouver, Canada), 2 mM L-Glutamine (Sigma-Aldrich, St. Louis, Missouri, United States), 1 mM streptomycin/penicillin, 0.1 mM 2-mercaptoethanol, 0.02 mg/mL human Low Density Lipoprotein (STEMCELL Technologies, Vancouver, Canada). .. The SFM was then filtered and supplemented with a physiological growth factor cocktail (STEMCELL Technologies, Vancouver, Canada) consisting of 0.05 ng/mL human recombinant LIF, 1 ng/mL G-CSF and 0.2 ng/mL SCF/GM-CSF/MIP-α/IL-6.

Article Title: Nuclear-to-cytoplasmic Relocalization of the Proliferating Cell Nuclear Antigen (PCNA) during Differentiation Involves a Chromosome Region Maintenance 1 (CRM1)-dependent Export and Is a Prerequisite for PCNA Antiapoptotic Activity in Mature Neutrophils
Article Snippet: .. CD34 cells were next isolated using the CD34 progenitor cell isolation kit (Miltenyi Biotec, Cologne, Germany) andwere culturedwith stemcell factor (SCF, 100ng/ml), IL-3 (10 ng/ml), and IL-6 (100 ng/ml) in Iscove’s medium Dulbecco’s modified (Invitrogen) supplemented with 15% BIT 9500 (BSA-insulin-transferrin, Stem Cell Technologies), antibiotics (penicillin 100 units/ml, streptomycin 100 g/ml), and L-glutamine 2 mM for 7 days. .. CD36 cells were next negatively selected by incubation with monoclonal IgG1 anti-CD36 antibody (BD Biosciences) followed by anti-mouse IgG1 antibody coupled to magnetic beads (Miltenyi Biotec).

Recombinant:

Article Title: A novel, highly potent and selective phosphodiesterase-9 inhibitor for the treatment of sickle cell disease
Article Snippet: .. CD34 + cells were were cultured in the presence of 15% BIT 9500 [mixture of bovine serum albumin (BSA) + insulin + transferrin from Stem Cell Technologies], 100 U/mL penicillin-streptomycin, 2 mM L-glutamine, 10 ng/mL recombinant human (rh) IL-3 (Peprotech), 100 ng/mL rhIL-6 (Peprotech), and 100 ng/mL rhSCF (Peprotech) for seven days and then CD36 + cells, isolated and cultured in media containing 100 ng/mL rhSCF, 10 ng/mL rhIL-3 and 2 UI/mL erythropoetin (Cilag, France) supplemented with dimethyl sulfoxide (DMSO), 30 μM HU or 10 μM IMR-687 for five days, at which point the HbF + erythroid cells (LD−/GPA + /Band3 + ) was determined by FACS. .. HbSS-Townes mice on a 129/B6 background (Jackson Laboratory, Bar Harbor, ME, USA; 10-12 weeks old, n=7 per group) were dosed daily by gavage with vehicle (polyethylene glycol in water 1:3), 50 or 25 mg/kg of HU, or 30 mg/kg of IMR-687.

Isolation:

Article Title: A novel, highly potent and selective phosphodiesterase-9 inhibitor for the treatment of sickle cell disease
Article Snippet: .. CD34 + cells were were cultured in the presence of 15% BIT 9500 [mixture of bovine serum albumin (BSA) + insulin + transferrin from Stem Cell Technologies], 100 U/mL penicillin-streptomycin, 2 mM L-glutamine, 10 ng/mL recombinant human (rh) IL-3 (Peprotech), 100 ng/mL rhIL-6 (Peprotech), and 100 ng/mL rhSCF (Peprotech) for seven days and then CD36 + cells, isolated and cultured in media containing 100 ng/mL rhSCF, 10 ng/mL rhIL-3 and 2 UI/mL erythropoetin (Cilag, France) supplemented with dimethyl sulfoxide (DMSO), 30 μM HU or 10 μM IMR-687 for five days, at which point the HbF + erythroid cells (LD−/GPA + /Band3 + ) was determined by FACS. .. HbSS-Townes mice on a 129/B6 background (Jackson Laboratory, Bar Harbor, ME, USA; 10-12 weeks old, n=7 per group) were dosed daily by gavage with vehicle (polyethylene glycol in water 1:3), 50 or 25 mg/kg of HU, or 30 mg/kg of IMR-687.

Article Title: Nuclear-to-cytoplasmic Relocalization of the Proliferating Cell Nuclear Antigen (PCNA) during Differentiation Involves a Chromosome Region Maintenance 1 (CRM1)-dependent Export and Is a Prerequisite for PCNA Antiapoptotic Activity in Mature Neutrophils
Article Snippet: .. CD34 cells were next isolated using the CD34 progenitor cell isolation kit (Miltenyi Biotec, Cologne, Germany) andwere culturedwith stemcell factor (SCF, 100ng/ml), IL-3 (10 ng/ml), and IL-6 (100 ng/ml) in Iscove’s medium Dulbecco’s modified (Invitrogen) supplemented with 15% BIT 9500 (BSA-insulin-transferrin, Stem Cell Technologies), antibiotics (penicillin 100 units/ml, streptomycin 100 g/ml), and L-glutamine 2 mM for 7 days. .. CD36 cells were next negatively selected by incubation with monoclonal IgG1 anti-CD36 antibody (BD Biosciences) followed by anti-mouse IgG1 antibody coupled to magnetic beads (Miltenyi Biotec).

FACS:

Article Title: A novel, highly potent and selective phosphodiesterase-9 inhibitor for the treatment of sickle cell disease
Article Snippet: .. CD34 + cells were were cultured in the presence of 15% BIT 9500 [mixture of bovine serum albumin (BSA) + insulin + transferrin from Stem Cell Technologies], 100 U/mL penicillin-streptomycin, 2 mM L-glutamine, 10 ng/mL recombinant human (rh) IL-3 (Peprotech), 100 ng/mL rhIL-6 (Peprotech), and 100 ng/mL rhSCF (Peprotech) for seven days and then CD36 + cells, isolated and cultured in media containing 100 ng/mL rhSCF, 10 ng/mL rhIL-3 and 2 UI/mL erythropoetin (Cilag, France) supplemented with dimethyl sulfoxide (DMSO), 30 μM HU or 10 μM IMR-687 for five days, at which point the HbF + erythroid cells (LD−/GPA + /Band3 + ) was determined by FACS. .. HbSS-Townes mice on a 129/B6 background (Jackson Laboratory, Bar Harbor, ME, USA; 10-12 weeks old, n=7 per group) were dosed daily by gavage with vehicle (polyethylene glycol in water 1:3), 50 or 25 mg/kg of HU, or 30 mg/kg of IMR-687.

other:

Article Title: Glioma Big Potassium Channel Expression in Human Cancers and Possible T Cell Epitopes for Their Immunotherapy
Article Snippet: For continuous expansion, half of this medium was replaced every other day, and the cultures were passaged every 7 d or when confluent using nonenzymatic cell dissociation solution (Sigma-Aldrich).

Cell Isolation:

Article Title: Nuclear-to-cytoplasmic Relocalization of the Proliferating Cell Nuclear Antigen (PCNA) during Differentiation Involves a Chromosome Region Maintenance 1 (CRM1)-dependent Export and Is a Prerequisite for PCNA Antiapoptotic Activity in Mature Neutrophils
Article Snippet: .. CD34 cells were next isolated using the CD34 progenitor cell isolation kit (Miltenyi Biotec, Cologne, Germany) andwere culturedwith stemcell factor (SCF, 100ng/ml), IL-3 (10 ng/ml), and IL-6 (100 ng/ml) in Iscove’s medium Dulbecco’s modified (Invitrogen) supplemented with 15% BIT 9500 (BSA-insulin-transferrin, Stem Cell Technologies), antibiotics (penicillin 100 units/ml, streptomycin 100 g/ml), and L-glutamine 2 mM for 7 days. .. CD36 cells were next negatively selected by incubation with monoclonal IgG1 anti-CD36 antibody (BD Biosciences) followed by anti-mouse IgG1 antibody coupled to magnetic beads (Miltenyi Biotec).



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